commercial human myoc  (OriGene)

Journal: Ophthalmology. Glaucoma

Article Title: Histochemical Analysis of Glaucoma Caused by a Myocilin Mutation in a Human Donor Eye

doi: 10.1016/j.ogla.2018.08.004

Figure Lengend Snippet: Brightfield microscopic images show H&E staining of the iridocorneal angle in an eye with a MYOC Tyr437His mutation (A) and three normal control eyes (B-D). Intense MYOC immunoreactivity (red) was observed in the trabecular meshwork of the eye with a MYOC Tyr437His mutation (E) No MYOC immunoreactivity was observed in normal control 1 (F), an age-matched donor with no known eye disease whose eye was identically fixed in 10% formalin to match fixation of the MYOC Tyr437His eye. Four additional normal control eyes from donors with no known ocular disease were previously fixed in 4% paraformaldehyde and were available for use as an additional set of controls. No MYOC immunoreactivity was observed in three of the four additional control eyes, as shown with a representative image from normal control 2 (G). One of the four additional control eyes, normal control 3, exhibited minimal positive MYOC labeling (H). The MYOC Tyr437His eye and all of the normal control eyes show collagen IV immunoreactivity (green) of the trabecular meshwork (I-P). At higher magnification, collagen IV is seen deposited along the beams of the trabecular meshwork (M-P), with minimal to no co-localization with MYOC in the MYOC Tyr437His eye (M) or in normal control 3 (P). Artifactitious tissue separation during processing is denoted by an asterisks. Black and white arrows point to the trabecular meshwork. Cell nuclei are labeled with DAPI (blue). TM, trabecular meshwork; AC, anterior chamber; CB, ciliary body. Scale bars = 100μm.

Article Snippet: Specificity of the MYOC antibody was confirmed by preincubation of the antibody with a 10X excess of recombinant MYOC protein (H00004653-P01, Novus biologicals, San Diego, CA) ( Supplemental Figure 1 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1. caption a7 caption a8 Immunohistochemical detection of MYOC protein in the trabecular meshwork of an eye with a MYOC Tyr437His mutation.

Techniques: Staining, Mutagenesis, Control, Labeling

Journal: Ophthalmology. Glaucoma

Article Title: Histochemical Analysis of Glaucoma Caused by a Myocilin Mutation in a Human Donor Eye

doi: 10.1016/j.ogla.2018.08.004

Figure Lengend Snippet: Confocal microscopy shows MYOC immunoreactivity in the trabecular meshwork of the eye with a MYOC Tyr437His mutation that co-localizes with two markers for trabecular meshwork cells, wheat germ agglutinin (WGA) lectin (A-D), and concanavalin (ConA) lectin (E-H). Co-labeling suggests localization of MYOC within trabecular meshwork cells. MYOC immunoreactivity also co-localizes with GRP78/BiP, a marker of ER stress (I-L) Cell nuclei are labeled with DAPI (blue). Scale bars, 10 μm.

Article Snippet: Specificity of the MYOC antibody was confirmed by preincubation of the antibody with a 10X excess of recombinant MYOC protein (H00004653-P01, Novus biologicals, San Diego, CA) ( Supplemental Figure 1 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1. caption a7 caption a8 Immunohistochemical detection of MYOC protein in the trabecular meshwork of an eye with a MYOC Tyr437His mutation.

Techniques: Confocal Microscopy, Mutagenesis, Labeling, Marker